[Steered Molecular Dynamics Simulation Study of Quantified Effects of Point Mutation Induced by Breast Cancer on Mechanical Behavior of E-Cadherin].

Title[Steered Molecular Dynamics Simulation Study of Quantified Effects of Point Mutation Induced by Breast Cancer on Mechanical Behavior of E-Cadherin].
Publication TypeJournal Article
Year of Publication2018
AuthorsAzadi S, Tafazzoli-Shadpour M, Omidvar R
JournalMol Biol (Mosk)
Volume52
Issue5
Pagination836-845
Date Published2018 Sep-Oct
ISSN0026-8984
KeywordsAntigens, CD, Breast Neoplasms, Cadherins, Cell Adhesion, Humans, Molecular Dynamics Simulation, Point Mutation, Protein Structure, Tertiary
Abstract

E-cadherin is a member of the cadherin family that plays a key role in the formation of cell-cell adhesion among epithelial tissues. Point mutations are one of the structural abnormalities of E-cadherin in human carcinomas. Such abnormalities can alter mechanical properties of proteins that play an important role in their biological activities. To determine the impact of point mutations on protein mechanical properties, the second fragment of extracellular domain of E-cadherin was modeled using steered molecular dynamics simulations. The molecular dynamics modeling included application of tensile forces in both constant velocity and constant force modes to examine the effects of Met282 to He and Asn315 to Ser mutations on mechanical behavior of protein structure. The stabilities of the wild type and mutant structures were also obtained by the protein design foldX algorithm. Results confirmed the lower stability of the mutant domains compared to the wild type. The mutated proteins displayed softer behavior than the reference protein and their stiffness decreased by up to 34%. Our findings suggest that local changes in molecular structure due to mutations may lead to noticeable alterations in mechanical properties within the entire domain. Since the function of protein is related to its structure, these changes may influence the function of the protein.

DOI10.1134/S002689841805004X
Alternate JournalMol. Biol. (Mosk.)
PubMed ID30363059